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Probably not: https://support.bioconductor.org/p/74700/
FourCseq does not support working without replicates. If you are working without replicates, 4C-ker and w4Cseq softwares could work for you. The authors of w4Cseq have a good tutorial explaining how to use it
w4Cseq : http://w4cseq.wglab.org/