Differential expression analysis of normalized expression of RNA seq data

Dear All,

I have a normalized expression table of RNA seq data. I normally use DESeq2 but it needs raw count table not normalized data. Which R package I can use to analyse a Normalised table?

Best,

rna-seq

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updated 8.0 years ago by Benn

• written 8.0 years ago by Javad

Normalized by what method? Are there replicates?

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written 8.0 years ago by ATpoint

This post from the bioconductor support site maybe of help: https://support.bioconductor.org/p/56275/

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written 8.0 years ago by ejm32

1 answer

None. There is no package that is able to do (sound) statistical analysis of normalized RNAseq data. Get read count data, and do what you normally do with DEseq2 or limma voom, etc.

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written 8.0 years ago by Benn

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