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Variance stabilizing transformation

Hi, Does anyone know the reasons for this type of an error while trying to transform count data of 4C-seq experiment using FourCseq package:

Error in getZScores(fc, removeZeros = TRUE) : Failed to estimate the parameters of the Variance stabilizing transformation (VST).

Or in general- what is the reason for failure in estimation of VST parameters?

Thanks, Tal

fourcseq variance stabilizing transformation

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