Genome Analysis
Hello friends
I am looking for some sort of protocol for genome analysis ,starting with assembly of contigs. i have a nucleotide sequencing data some fungal species and now im interested in performing a genome analysis of the data,i have a prior knowledge of protein anlaysis but i have never performed a genome analysis,
i have read about genome analysis but there does'nt seems to b a prpoer methodolgy to it except 1.Assembly of genomic sequence 2.predicting repeated sequences 3. Gene Prediction 4. EST and CDNA Sequences analysis 5. GENOME ANNOTATION
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And once you have those contigs, what exactly is it that you're seeking to accomplish? Do you wish to identify genes, genes of a particular type, or compare genomes. You may just want to know the species distribution, say in a metagenomic sample. An objective of your proposed analysis needs to be stated in order to see some useful responses.
TLI - Too little information
“Before beginning a Hunt, it is wise to ask someone what you are looking for before you begin looking for it.” W. t. Pooh
I'm going to suggest that you read this, then come back with a more specific question: Ten Simple Rules for Getting Help from Online Scientific Communities