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NCBI error report

Hi all, I am getting this Message ID#29 Error: Query string not found in the CGI context; when I am trying to align two or more seq in BLASTP.

Kindly answer.

blastp alignment

You're going to have to provide more information. Is this via the NCBI website? What's are the sequences you're trying to blast? What settings are you using?

Yes, I am trying it from NCBI website with default settings. Thanks for reply

2 answers

if you are not using standalone.

try NCBI COBALT which is meant for multiple sequence alignment.

http://www.ncbi.nlm.nih.gov/tools/cobalt/cobalt.cgi?LINK_LOC=BlastHomeLink

or you can use culstal omega of EMBL-EBI

http://www.ebi.ac.uk/Tools/msa/clustalo/

I ran into this error today, and I found out the reason for the error.

To resolve:

Just untick the Align two or more sequences box.

My blast search then worked like a charm!!

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