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Why I Am Getting This Error " No Alias Or Index File Found For ..... Database"

I format my yeast.aa amino acid with this command 

makeblastdb -dbtype prot -in yeast.aa -hash_index

it created several files then i run this query command with blastp

blastp -query reptiles.fasta -db yeast

which throws error saying

no alias or index file found for ......

please resolve my issue

actually i want to align two seq on my machine using standaloneblast+

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updated by Neilfws Neilfws • written by bioinformaticswelkinakash • bioinformaticswelkinakash

It doesn't find your database. So where is it?

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written by Michael Dondrup Michael Dondrup

sir i have placed yeast.aa in same folder where after executing makeblast all file is also kept , ifact all file in same folder from all exe to db files

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written by bioinformaticswelkinakash • bioinformaticswelkinakash

try blastp -query reptiles.fasta -db yeast.aa if that doesn't work locate the full path to yeast.aa and try that: blastp -query reptiles.fasta -db /path/to/yeast.aa

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written by Michael Dondrup Michael Dondrup

1 answer

If you don't specify a name for your db, then it will be exactly the same as the input file, i.e. in this case "yeast.aa". The files in your directory are called yeast.aa.nhd, yeast.aa.nhi, etc., aren't they?

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written by 5heikki 5heikki

yes they are. but why I am getting this error message " No alias or index file found for protein database [yeast] in search path [c::\user\welkin_akash\Desktop\Job portal\vvk sir\bin; ;]

how to create that index file

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written by bioinformaticswelkinakash • bioinformaticswelkinakash

The error is because in your blastp command line you call the database "yeast", when it should be called "yeast.aa".

Alternatively, use "-out yeast" in the makeblastdb command line, to name the database "yeast".

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written by Neilfws Neilfws

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