Hi All, Edited title of post to include "not perl," as this post was commented as a duplicate of another post which I found unhelpful. …

I'm trying to design some primers myself because all of the primer packages I've found are either web-based, are not very user friendly, cannot handle …

Hi all, I am relatively new to much of the world of bioinformatics and am struggling with finding my degenerate primer sequences. I have some …

Hi, I am trying to find the best match of the primer sequence which is 20bp long from a multi fasta file consisting 3 sequences …

Hello there, Is there a certain software / script that you can recommend for looking for degenerate sequences in multi-fasta file? I know that primer-blast …

I am using python 3 and Biopython 1.72 . I have been reconstructing a parsimony tree using my aligned protein sequence file with biopython which …

Is there a way to determine the direction of a beta stand? I know that it is possible because pymol shows the direction of each …

I would like to design a number of arbitrary degenerate primers (primers with variable bases, e.g. **NGATWGCTSATNGC**) for a [TAIL-PCR][1]. I would like to be …

A member of our lab is using [Primer-Blast][1] to find what DNA her pairs of primers amplify in the NT nucleotide database. She designed degenerated …

Hi All I have been trying to write a script to take IUPAC compliment primers and pull out the corresponding subsequence from a full length …