Biostar Beta. Not for public use.
Question: What is the difference between narrow peak, Broad peak and a gapped peak of a chip-seq experiment and is it okay to use all of these regions for downstream analysis
5
Entering edit mode

Many publicly available data sets (road map epigenomics data in particular) has three kinds of peaks (based on different parameters used to do peak calls in MACS2) namely "narrow" peaks, "broad" peaks and "gapped" peaks. I was wondering how are these three types of peaks different from each other and when doing downstream analysis using this kinds of peaks data would it be ok to use bedtools to merge these peak regions and use that instead of doing the analysis individually on all the different types of peaks.

ADD COMMENTlink 4.4 years ago Saad Khan • 330 • updated 4.4 years ago TriS ♦ 3.8k
7
Entering edit mode

you can find more info on the UCSC site and forum .

narrowpeaks can be generally called for TF, since the region bound is pretty much limited. broadpeaks are better for histone modifications or histone modifiers since the regions can be much wider. I'm not totally sure about gapped peaks but looks like you can used them when combiing regions that contain gaps (yeah, intuitive...).

and yes, you can use them for downstream analysis.

why would you want to merge them tho? that can give you gigantic peaks with basically no information. if you look at the co-binding of multiple TFs or histone marks, then I'd go for overlaps.

ADD COMMENTlink 4.4 years ago TriS ♦ 3.8k
Entering edit mode
0

So I am looking at specific regions of Histone Modifications in Human data and these peaks may have overlapping regions in that range. So in your opinion if I am looking at histones I should only look at Broad peaks. Or look at each of those peaks separately.

ADD REPLYlink 4.4 years ago
Saad Khan
• 330
Entering edit mode
0

I have some similar analysis need to do. Have you finished it? How did you cooperate your TF data with your data for histone modification?

ADD REPLYlink 3.3 years ago
sophialovechan
• 40
Entering edit mode
0

I am looking at transcription factor binding sites and the patterns of histone modifications around those sites I was wondering if I should use different peaks(broad/narrow/gapped) for different types of histone modifications??

ADD REPLYlink 4.3 years ago
Saad Khan
• 330
Entering edit mode
0

for histone mods I would use broad peaks, independently from the modification

ADD REPLYlink 4.3 years ago
TriS
♦ 3.8k
Entering edit mode
2

Then why do the Roadmap data have narrow peaks,broad peaks and gapped peaks for different kinds of modifications?

ADD REPLYlink 4.3 years ago
Saad Khan
• 330
Entering edit mode
0

I read that you could also call peaks in two runs, one with MACS2 narrow and another one with MACS2 broad, and then merge the output to have a mixed-type calling. Is this a correct way to do it, or is it better to call the mixed-type factors with other peak callers, such as PeakRanger's BCP, CCAT and Ranger (or even others)? (I would use this for a histone mark that I suspect might be having narrow behavior and broad as well)(PS: I assume with gapped peaks you mean mixed type).

ADD REPLYlink 11 months ago
msimmer92
• 180

Login before adding your answer.

Similar Posts
Loading Similar Posts
Powered by the version 2.0