Is there any way to calculate the mean coverage of each amplicon in a whole run (in 16 or 32 samples)?

I have run Velvet and ABySS using default parameters on my pair-end and single-end datasets, I have two questions related to the generated contigs. 1. …

How to calculate coverage in targeted sequencing using Lander/Waterman equation?

Why Velvet and ABySS, the de Bruijn graph assemblers produce greater number of contigs than any other assembler? Ideally, a good assembler should generate less …

Why Ray assembler takes more time to assemble reads as compare to other de novo assembler like ABySS, Velvet, SGA or PERGA?

What is the difference between Genome fraction and Genome coverage while aligning contigs on reference genome in de novo genome assembly?

Dear Friends, I am a beginner in the field of De novo Genome Assembly. I tried to understand coverage cut off and expected coverage parameters …

<p>I am completely new to Velvet, and I am mainly using &quot;Vague&quot; GUI to work with Velvet. I have been trying to experiment a lot …

<p>What is N50 in de novo genome assembler &quot;Velvet&quot;? and why we calculate it?</p>

<p>Having been really pleased with results from the Ray assembler I was a little disappointed to see that there are no coverage statistics in the …