Hey everyone I need some help with SAMtools (v 1.3.1) and such. I have 2 files that I want to align, with the ultimate goal …

Hello everyone, I'm doing de novo assembly from 150 bp whole genome shotgun reads. My pipeline is: 1. Trimmomatic (trimming and quality filtering) 2. SOAPdenovo2 …

Hi all, I am not clear about differences between de novo assembly and genome resequencing. I know that if there is no reference genome of …

Why do we required de novo sequencing if almost all the organisms are being sequenced? Why Don't we only focus on reference based sequencing?

What is the difference between Exome sequencing versus RNA sequencing ? What type of de novo assemblers should be use for exome sequencing reads and …

<p>What is N50 in de novo genome assembler &quot;Velvet&quot;? and why we calculate it?</p>

Dear Sir/Madam, I have single end data (Ion Torrent) and de novo assembly performed with MIRA (660 contigs) and SPADES (753 contigs). I just blasted …

<p>Is there any open source assembly softwares that takes in de novo assembly generated contigs and reference genome to produce bigger contigs( reference guided de …

<p>All, I am curious whether anyone out there has a method for assessing the quality and accuracy of de novo genome assemblies? I am currently …

<p>Hi</p> <p>Are there existing tools for visualizing RNA-Seq splicing graphs from de novo assembly without a reference genome?</p> <p>What I'd like is something that can …