Dear all, I am trying to do a short-long read hybrid de novo assembly on the MiSeq 2x75 paired end data and TruSeq long reads …

I have run Velvet and ABySS using default parameters on my pair-end and single-end datasets, I have two questions related to the generated contigs. 1. …

I have a set of paired end reads from an unknown species. I want to assemble them to find the main genome. I have a …

Why Ray assembler takes more time to assemble reads as compare to other de novo assembler like ABySS, Velvet, SGA or PERGA?

What is the difference between Exome sequencing versus RNA sequencing ? What type of de novo assemblers should be use for exome sequencing reads and …

<p>Hello everyone,</p> <p>I am trying to assemble a genome full of repetitive sequences. So far I tested SOAPdenovo2, platanus, (dip)SPAdes and velvet. I noticed a …

<p>What is N50 in de novo genome assembler &quot;Velvet&quot;? and why we calculate it?</p>

There are numerous de novo assemblers such as ABySS, SPAdes, AMOS, etc. And there is only one transcript assembler that I know of: **Inchworm** in …

<p>Is there any tool to automatically clip the flanking sub-sequences of contigs (beginning and end of each contig) that overlap with other contigs? Is there …

<p>I have got overlapping MiSeq 2x250bp reads (after merging single-end 400-450bp). The genome size is ~20Mb. I think de Bruijn graph based assemblers is not …