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converting the format of read files in GEO datasets (SRA) to fasta and fatsq

Hey guys,

I have an accession number, how I can convert the sra to fasta in Windows?

rna-seq

1 answer

You can use SRAtools for this purpose. I think it is available for both Windows and Linux. Once installed, you can download/convert sra files to fastq as follows:

fastq-dump myfile.sra #for single end
fastq-dump --split-files myfile.sra #if paired end

Thank you very much

For converting to fastq directly

 fastq-dump --split-files --gzip

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