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how to make regulatory network from microarray data

India

Hiii, I am a beginner, and doing my masters degree research work. I am currently working on gene expression data for saccharomyces cerevisiae with the help of microarray. I want to make a regulatory network of genes which are present in my data. but I am struck as I don't know how to make network from a microarray gene expression data. I installed the GeneMania app in the cytoscape and didn't get any idea how to proceed.

gene genome
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updated by Ram Ram • written by goforitpeehu • goforitpeehu

Cytoscape, did you read the manual. It has so many plug-ins. more you explore, more you get into the networks. :)

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written by Manvendra Singh Manvendra Singh

Personally, I'd not be very confident in such analysis basing on gene expression data alone. It shows only potential changes on the protein level. Tools like GeneMania will find plethora of interactions but it should be interpreted with great caution. @goforitpeehu Try to find some evidence at the protein level (eg. using data from public repositories like, arrayexpress, pride, geo).

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written by boczniak767 boczniak767

I am a beginner, and doing my masters degree research work.

Do you want to pinpoint a fundamental flaw in your educational system?

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updated by Ram Ram • written by Michael Dondrup Michael Dondrup

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