Dear all, I am using Stringtie to reconstitute transcriptome using non stranded RNA-seq data. Can anyone tell me, strand assignment by stringtie is really reliable …

Dear All, I need to create my own anti-microbial gene in swine. But I'm not sure how can I know that my database is cover …

According to WGS data, I finally obtain my vcf files (SNP and INDEL calling) by GATK4. Now, I'd like to obtain my personalized genome sequence …

Dear All, I have a VCF file containing 60 samples. I want to calculate MAF for each variant with respect to 60 samples present in …

Dear all Can anybody tell me how to get interacting pathways form KEGG databases. I have a list of KO numbers with associated multiple pathways. …

Dear all Can anybody tell me how can I get co-crystallized structure ID from RCSB PDB or Uniprot. I have 88 PDBIDs and its corresponding …

Dear All, I would like to know How Can I signUp DNAStar page, Coz I need to download the key fot trail version. I tried …

Dear all, I am working with TCGA pancancer gene expression level 3 data. I found that there are some genes expression levels are negative. Could …

Dear all, Could anyone please tell me how do I carry out a blast of a sequence against a large number of results at once? …

Dear all, Is there a tool I can use for mining for a particular gene in metaHIT? Would greatly appreciate any help, Thanks.