How to do automated blast with mulitple sequences and BLAST each one individually???
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9.7 years ago
jeccy.J ▴ 60

Is there an automated program that can take mulitple sequences and BLAST each one individually???

Blast • 2.0k views
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You might want to add more information to your question. Do you want to do this in parallel? Do you want individual output files for each query? Is there a reason why you can't or do not want to parse a results file with multiple queries in it?

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I want individual outputfile of each query. I want to do locally.

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Can you explain why you want separate output files for each query? It's hard to imagine why this is necessary, given that parsing a single output file is quite easy.

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9.7 years ago
pld 5.1k

Yes, BLAST will take a fasta file with multiple sequences and search each one against the target database.

I would set blast to tabular output, and write a simple script to chop it into single results.

The other option is to split the input fasta file into a single file per query sequence, and then run blast on each input file.

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