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Limit : this week
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174 results • Page
2 of 4
Sort: replies
Rank
Views
Votes
Replies
3
votes
3
replies
306
views
Is it possible to bulk download files from GEO repository?
RNA-seq
Galaxy
Tanscriptomics
updated 3 days ago by
noodle
▴ 580 • written 3 days ago by
WUSCHEL
▴ 750
3
votes
3
replies
298
views
genome assembly records not present in assembly_summary.txt
ncbi
bacteria
assembly
updated 6 days ago by
GenoMax
142k • written 6 days ago by
sapuizait
▴ 10
0
votes
3
replies
264
views
Landmark gene selection in L1000.
L1000
landmark-gene
cmap
updated 2 days ago by
GenoMax
142k • written 3 days ago by
kim
• 0
1
vote
3
replies
253
views
DESeq2 processing problems
deseq2
R
updated 1 day ago by
ATpoint
82k • written 1 day ago by
jagdish7921
• 0
0
votes
3
replies
324
views
CWl and toil singularity image e.g busybox? Thank you
toil
singularity
updated 3 days ago by
Ram
43k • written 4 months ago by
Fadi
• 0
4
votes
3
replies
354
views
Source other conda environments in a nextflow pipeline when nextflow itself is in a conda environment?
hpc
conda
nextflow
updated 5 days ago by
Arup Ghosh
3.2k • written 6 days ago by
chaco001
▴ 40
5
votes
3
replies
249
views
6 follow
Integrate transcriptomic data and proteomics data.
OMICS
updated 2 days ago by
Lluís R.
★ 1.2k • written 2 days ago by
이민경[학생](대학원 융합의과학과)
• 0
1
vote
3
replies
294
views
How to assign cell types after integration in scRNA
scRNA-seq
updated 4 days ago by
ATpoint
82k • written 4 days ago by
Francesco
▴ 10
1
vote
3
replies
370
views
PCA plot
DESeq2
PCAplot
updated 4 days ago by
LauferVA
4.2k • written 7 days ago by
Aaliya
▴ 10
1
vote
3
replies
379
views
Help understanding how KEGG Ortholog `K00004 ` has 3 ECs associated with it (EC:1.1.1.4, 1.1.1.-, 1.1.1.303)?
ontology
metagenomics
database
enzymes
genomics
updated 4 days ago by
Mensur Dlakic
★ 27k • written 5 days ago by
O.rka
▴ 710
1
vote
3
replies
89
views
How to access TCGA samples that were treated with a specific drug?
TCGA
RNA-seq
updated 40 minutes ago by
GenoMax
142k • written 4 hours ago by
Qroid
▴ 40
2
votes
3
replies
349
views
How to establish haplotype-specific gene expression levels
RNA-seq
Haplotype
updated 6 days ago by
dsull
★ 5.9k • written 23 days ago by
javanokendo
▴ 60
0
votes
3
replies
726
views
Why cd-hit-est not work when sequence identity threshold<0.95?
genome
cluster
sequence
updated 2 days ago by
weidonglu
• 0 • written 21 months ago by
JZX
• 0
0
votes
3
replies
261
views
KEGG Pathways
r
RNA-seq
updated 2 days ago by
Ram
43k • written 2 days ago by
Sudip
• 0
0
votes
3
replies
297
views
Highest variable features in single cell data
single-cell
updated 6 days ago by
bk11
★ 2.4k • written 7 days ago by
Kazo
▴ 10
0
votes
3
replies
203
views
Software to separate reads from different individuals
software
development
nanopore
updated 3 days ago by
GenoMax
142k • written 3 days ago by
njornet
▴ 20
1
vote
3
replies
253
views
What does 'bundle_uuid' refer to in this metadata sheet?
SRA
illumina
GEO
metadata
updated 2 days ago by
GenoMax
142k • written 2 days ago by
jeffrey.maurer.informatics
• 0
1
vote
3
replies
78
views
DiffBind: no peaks in DBA
MACS2
DiffBind
ChIP-seq
updated 32 minutes ago by
jared.andrews07
★ 16k • written 4 hours ago by
yvonneh
▴ 10
0
votes
3
replies
375
views
why renaming Idents in Seurat object doesn't work?
Seurat
RenameIdents
R
2 days ago by
Assa Yeroslaviz
★ 1.8k
3
votes
3
replies
300
views
Sequence read length shorter than flow cell specification
illumina
NGS
sequencing
updated 6 days ago by
swbarnes2
14k • written 7 days ago by
M
• 0
0
votes
2
replies
195
views
What should I consider as FASTA for dataset?
PDB
FASTA
3 days ago by
Nafi
• 0
0
votes
2
replies
251
views
Correct way to compare multiple treaments between RNA-Seq samples using edgeR?
RNA-Seq
edgeR
5 days ago by
Guille
• 0
3
votes
2
replies
327
views
what is the purpose of indexing the reference genome (Kallisto)
indexing
Kallisto
updated 6 days ago by
dsull
★ 5.9k • written 6 days ago by
Aaliya
▴ 10
1
vote
2
replies
331
views
ScRNAseq-How to correctly choose cell type marker genes
cellAssign
cell-markers
4 days ago by
Francesco
▴ 10
1
vote
2
replies
215
views
POSSUM not working due to incompatible pssm file
fasta
pse-pssm
pssm
POSSUM
15 hours ago by
rianna.collins
• 0
1
vote
2
replies
142
views
Odd alignment question/finding
Alignment
updated 13 hours ago by
ATpoint
82k • written 18 hours ago by
poordumbsillyidiot
• 0
1
vote
2
replies
37
views
Converting CRAM to FastQ
GATK
samtools
cram
SamToFastq
fastq
34 minutes ago by
Maverick
▴ 10
0
votes
2
replies
190
views
How to update R on ubuntu
installation
update
R
apt-get
3 days ago by
Bosberg
▴ 50
0
votes
2
replies
148
views
Longitudinal analysis of subpopulations: which approach is better?
differential-expression
DEG
model
3 days ago by
Lluís R.
★ 1.2k
1
vote
2
replies
228
views
Do I need to go back and filter my long-reads?
alignment
nanopore
filtering
QC
ONT
updated 5 hours ago by
Ram
43k • written 17 days ago by
eesiribloom
▴ 80
2
votes
2
replies
181
views
MA plot of shrunken fold change
MA-plot
Deseq2
updated 2 days ago by
ATpoint
82k • written 2 days ago by
Sudip
• 0
4
votes
2
replies
394
views
Missing protein (VEGF-A) in String db
VGFA
stringdb
updated 6 days ago by
shalespringer
• 0 • written 12 weeks ago by
Phenylananin
▴ 20
0
votes
2
replies
86
views
How does gene length effect the number of reads mapped
RNA-seq
CPM
sequencing
3 hours ago by
Chen
• 0
2
votes
2
replies
193
views
PDB related issue
rcsb
pdb
updated 3 days ago by
noodle
▴ 580 • written 4 days ago by
Nafi
• 0
3
votes
2
replies
288
views
ChIP-seq datasets: input samples omitted?
ChIP-seq
Normalization
NGS
updated 3 days ago by
i.sudbery
19k • written 6 days ago by
vanbelj
▴ 40
0
votes
2
replies
281
views
Rare Disease Variant Pathway Analysis
Pathway-analysis
updated 6 days ago by
Ram
43k • written 6 days ago by
The_PyPanda
▴ 10
0
votes
2
replies
50
views
Base Quality Score Recalibration with different reference genome
SNPs
GATK
BQSR
RNA-seq
2 hours ago by
dtnondorf
• 0
0
votes
2
replies
207
views
ERROR When Using Ensembl Plants Biomart Wget API
biomart
plant
ensembl
updated 2 days ago by
GenoMax
142k • written 2 days ago by
Dora
▴ 10
1
vote
2
replies
212
views
Need help for downloading arabdopsis thaliana reference genome fasta file and gtf file
Arabidopsis-thaliana
gtf
reference-genome
updated 2 days ago by
Ram
43k • written 2 days ago by
Ravita
• 0
0
votes
2
replies
340
views
Hide positions in alignment with 99% "–" characters to ignore single sequence insertions?
alignment
gaps
updated 5 days ago by
Jesse
▴ 740 • written 7 days ago by
Broccoli
• 0
3
votes
2
replies
359
views
Extract protein sequence
fasta
alignment
blast
2 days ago by
anna
▴ 20
2
votes
2
replies
239
views
p-value in CIBERSORT
CIBERSORT
p-value
1 day ago by
michelafrancesconi9
▴ 20
1
vote
2
replies
269
views
Minimum RAM and Storage requirement for creating PSSM using ncbi-blast-2.2.30+-x64-linux
blast
pssm
1 day ago by
Nafi
• 0
4
votes
2
replies
292
views
How to use limma to find differentially expressed genes in response to a continuous variable
limma
voom
R
3 days ago by
pairedttest
▴ 10
0
votes
1
reply
203
views
Finding batch and outlayers
Pca
updated 3 days ago by
christopher medway
▴ 450 • written 3 days ago by
Tigran
• 0
0
votes
1
reply
272
views
News:
FINAL CALL: 8th Berlin Summer School in NGS Data Analysis - Only a few last places available
DNA-seq
variant-calling
RNA-seq
illumina
transcriptomics
2 days ago by
David Langenberger
11k
1
vote
1
reply
95
views
What analysis suitable to identify similarly expressed genes between two samples (treated vs untreated). Contrary to DGE analysis,
Gene-Expression
RNA
NOISeq
DGE
updated 5 hours ago by
Ram
43k • written 8 hours ago by
alifafiq1
• 0
0
votes
1
reply
746
views
Can I run cellassign on samples independently if there is batch effect present?
RNA-Seq
R
cellassign
batch-effect
updated 4 days ago by
Francesco
▴ 10 • written 3.3 years ago by
gt
▴ 30
0
votes
1
reply
165
views
Downloading full alignments from Pfam
pfam
updated 4 days ago by
GenoMax
142k • written 4 days ago by
bef1
• 0
0
votes
1
reply
423
views
after selecting specific GO terms to plot, cneplot is not showing any color for genes, GO terms and only showing lines without nodes.
cneplot
updated 1 day ago by
Ram
43k • written 1 day ago by
Dr Huma Naz
• 0
174 results • Page
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Comment: Converting CRAM to FastQ
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Recent Replies
Comment: Extract gRNA sequence using cutadapt
by
gernophil
▴ 80
> Not every read in your data is going to match a guide. That is also true for sure. Let me clarify what I mean. For every read you should…
Answer: VG : No reference-sense paths available in the graph; falling back to generic pa
by
anovak
▴ 120
If your GFA has paths in it that are P lines with names that don't include a sample name, contig name, and separators, then those are what …
Comment: Extract gRNA sequence using cutadapt
by
GenoMax
142k
> if you look at a fastq file you should be able to tell for every read definitely, if it has a perfect match for a guide in it and what gu…
Answer: DiffBind: no peaks in DBA
by
jared.andrews07
★ 16k
Because `dba.analyze` is not meant to be run directly on a peaks file. Have you read the documentation? In addition, a ChIP-seq experiment…
Comment: Converting CRAM to FastQ
by
Maverick
▴ 10
Thank you so much! Will look it up right away.
Comment: Extract gRNA sequence using cutadapt
by
gernophil
▴ 80
> I don't think every guide is supposed to show up in these experiments as far as I have seen. You will get some guides with 0 counts as yo…
Comment: Converting CRAM to FastQ
by
GenoMax
142k
Use `samtools view ` for this conversion. See discussion in https://www.biostars.org/p/9592860/ Specifically @jkbonfield's comment here --…
Comment: How to access TCGA samples that were treated with a specific drug?
by
GenoMax
142k
No that was only from Breast cancer. You could try selecting all data and see if you are able to see all treatments in the set. No idea a…
Comment: Extract gRNA sequence using cutadapt
by
GenoMax
142k
I don't think every guide is supposed to show up in these experiments as far as I have seen. You will get some guides with 0 counts as you …
Comment: Extract gRNA sequence using cutadapt
by
gernophil
▴ 80
> Can you try lowering the k to k=9? Wouldn't that mess with the results since all my kmers are 20bp? > You don't have any duplicate sequ…
Comment: Provean help
by
Mensur Dlakic
★ 27k
> Else, I will happy to send over the data and kindly perform the analysis for me. This website is meant to provide advice, not service. I…
Comment: How to access TCGA samples that were treated with a specific drug?
by
Qroid
▴ 40
Thank you! Naive question, but does that list include all treatments in TCGA? I.e. is there anything missing from there, or that's it? Als…
Comment: Base Quality Score Recalibration with different reference genome
by
dtnondorf
• 0
Interesting! Thank you for such a quick response!
Comment: GATK genomicsDBimport intervals for WGS
by
Sd
• 0
I also have similar question. I sliced the genomic bed file with 50kb windows and 1kb padding into ~700 bed files; each bed file contains 9…
Comment: Base Quality Score Recalibration with different reference genome
by
GenoMax
142k
See answer here: https://www.biostars.org/p/9494503/#9494619 Heng Li said: https://twitter.com/lh3lh3/status/1410375781340135424 <!-- th…
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