19 days ago by
carlopecoraro2
★
2.5k
2
votes
0
replies
174
views
1
vote
2
replies
1.2k
views
24 days ago by
Zhenyu Zhang
★
1.2k
1
vote
2
replies
323
views
1
vote
0
replies
184
views
updated 12 days ago by
Ram
44k
•
written 12 days ago by
carlopecoraro2
★
2.5k
1
vote
2
replies
249
views
updated 8 days ago by
colindaven
6.4k
•
written 9 days ago by
Dr.
•
0
1
vote
0
replies
211
views
27 days ago by
carlopecoraro2
★
2.5k
0
votes
0
replies
161
views
updated 28 days ago by
Ram
44k
•
written 28 days ago by
carlopecoraro2
★
2.5k
0
votes
2
replies
462
views
0
votes
0
replies
199
views
0
votes
0
replies
136
views
0
votes
0
replies
155
views
26 days ago by
Amos Bairoch
▴
120
0
votes
0
replies
100
views
updated 7 days ago by
Ram
44k
•
written 8 days ago by
carlopecoraro2
★
2.5k
0
votes
0
replies
137
views
21 days ago by
carlopecoraro2
★
2.5k
0
votes
0
replies
138
views
17 days ago by
carlopecoraro2
★
2.5k
0
votes
0
replies
133
views
updated 26 days ago by
Ram
44k
•
written 27 days ago by
carlopecoraro2
★
2.5k
0
votes
0
replies
97
views
0
votes
0
replies
126
views
updated 1 day ago by
Ram
44k
•
written 3 days ago by
carlopecoraro2
★
2.5k
0
votes
0
replies
142
views
updated 20 days ago by
Ram
44k
•
written 20 days ago by
carlopecoraro2
★
2.5k
0
votes
0
replies
105
views
updated 8 days ago by
Ram
44k
•
written 8 days ago by
carlopecoraro2
★
2.5k
0
votes
0
replies
105
views
updated 6 days ago by
Ram
44k
•
written 6 days ago by
carlopecoraro2
★
2.5k
Recent Votes
Recent Locations •
All
Bologna, 1 minute ago
Poland, 6 minutes ago
Pakistan, 7 minutes ago
Greece, 8 minutes ago
India, 9 minutes ago
United States, 10 minutes ago
New Zealand, 11 minutes ago
Recent Awards •
All
Popular Question to
January
▴
30
Supporter to
txema.heredia
▴
130
Popular Question to
hellokwmin
•
0
Autobiographer to
mtabaka
▴
20
Popular Question to
DareDevil
★
4.3k
Recent Replies
Comment: when I use htslib to write a bam. Error of "truncated file" shows by samtools
by
GenoMax
142k
https://www.biostars.org/u/110878/ : Please accept this answer (green check mark) to provide closure to this thread.
Comment: bcl-convert cannot create output directory structure at the path specified
by
giulia.trauzzi
▴
10
Thanks! I am sure there are. Are these necessary to demultiplex the data though? I am assuming most demultiplexing tools do not require the…
Comment: Filtering reads that map to both references
by
GenoMax
142k
You can use `bbsplit.sh` from BBMap suite to map reads to multiple references and bin them accordingly. Take a look at the in-line help (`a…
Comment: bcl-convert cannot create output directory structure at the path specified
by
GenoMax
142k
Is that the only error? Can you provide the entire command line you are using?
> as a customer is interested in getting the fastq files f…
Comment: Filtering reads that map to both references
by
michael.flower.14
▴
180
Thanks Pierre. I'm working in a micromamba environment. I've got `jvarkit` installed. I updated your filter script as follows.
# Creat…
Comment: bcl-convert cannot create output directory structure at the path specified
by
giulia.trauzzi
▴
10
Hi, thanks for this. I checked the permissions of the folder and changed them with
chmod ugo+rwx /output_directory
This has changed …
Comment: RNA-Seq analysis
by
carlopecoraro2
★
2.5k
[Online course: ANALYSIS OF RNA SEQUENCING DATA WITH R/BIOCONDUCTOR][1]
----------
Dates
4-15 November 2024
[1]: https://www.physal…
Comment: How do i calculate the mean of triplicates in a data.frame based on pattern?
by
dariober
14k
I guess the OP doesn't want to hard-code `p767.AM` and `"AM"`?
Comment: bcl-convert cannot create output directory structure at the path specified
by
marco.barr
▴
140
I've never received this error before.. but you could try checking the permissions
Answer: How do i calculate the mean of triplicates in a data.frame based on pattern?
by
gglim
▴
140
```
library(dplyr)
df %>% rowwise() %>% mutate(p767.AM = mean(c_across(contains("AM"))))
```
Maybe this is what you're looking for?
Answer: RNA-Seq analysis
by
Michael
54k
I think the situation you are finding yourself in is quite common. On a general note, as you are at the beginning of your PhD program, you …
Answer: RNA-Seq analysis
by
Nat.Nataren
▴
30
We'll need a lot more information to help, you haven't given us much to work with.
What organism are you working in?
What is the experi…
Comment: Cut&Run and heatmap
by
gglim
▴
140
I'll take a look at these peaks in IGV with the signal files and set a threshold for filtering.
Comment: NCBI vs Ensembl - Ortholog genes Information
by
José
▴
10
Thanks for the answer and the advice.
Comment: NCBI vs Ensembl - Ortholog genes Information
by
José
▴
10
Thak you so much for your answer and advice.
Traffic: 2064 users visited in the last hour
Use of this site constitutes acceptance of our User Agreement and Privacy Policy.
Powered by the
version 2.3.6
version 2.3.6