Limit : this month
all time today this week this month this year
4 results • Page 1 of 1
Sort: Votes
Rank Views Votes Replies
25
votes
11
replies
3.8k
views
6 follow
Forum: Site Use Guidelines
meta biostars Guidelines
updated 28 days ago by 44k • written 14.6 years ago by 100k
19
votes
15
replies
2.3k
views
Forum: What is the amount of sequencing data produced annually?
data research sequencing
updated 1 day ago by • 0 • written 8 months ago by ▴ 70
6
votes
4
replies
460
views
Forum: Ideal PC configurations and operating system for bioinformatics laboratory
PC
20 days ago by Estevão ▴ 10
0
votes
0
replies
199
views
Forum: Improvement on automatic annotation of single-cell RNA-seq data from the literature using LLM
annotation scRNA llm
29 days ago by yxwucq • 0
4 results • Page 1 of 1
Recent Votes
Comment: Trimmomatic running but files containing purged reads are empty
C: Classifying normal vs cancer tissues from mutational data
Classifying normal vs cancer tissues from mutational data
Pymol Scripting Help
A: Not sure if pipe from bowtie2 to samtools is working?
Comment: FastQC interpretation - 16S sequencing
Comment: FastQC interpretation - 16S sequencing
Recent Locations • All
United States, just now
United States, 1 minute ago
United Kingdom, 1 minute ago
United States, 2 minutes ago
United States, 3 minutes ago
Ithaca, 3 minutes ago
United Kingdom, 4 minutes ago
Recent Awards • All
Teacher to Ashutosh Pandey 12k
Popular Question to Wilber0x ▴ 50
Popular Question to pinheirofabiano ▴ 20
Popular Question to andres_arroyo • 0
Popular Question to Tawny ▴ 180
Popular Question to anasjamshed ▴ 120
Student to Rafael Soler ★ 1.2k
Recent Replies
Comment: Trimmomatic running but files containing purged reads are empty by Wilber0x ▴ 50
Thanks for the advice, it seems like it is likely I have the incorrect adaptor sequences, though I am still surprised that no low quality r…
Comment: Trimmomatic running but files containing purged reads are empty by GenoMax 142k
> Why is trimmomatic not removing any reads? It is not mandatory that your data have extraneous/adapter sequence. If no extraneous sequenc…
Comment: FastQC interpretation - 16S sequencing by GenoMax 142k
Since a specific region is being amplified it is expected that many of the library fragments will have identical sequences. Thus the low nu…
Comment: FastQC interpretation - 16S sequencing by Christopher • 0
@genomax Thank you so much for replying. I was quite unsure about the qualities. One more question. There is no problem within nucleotide d…
Comment: FastQC interpretation - 16S sequencing by GenoMax 142k
> My specific question is about the feasibility of per base sequence content analysis and whether I should be concerned about a particular …
Comment: How to get the ratio of allele counts from GATK derived VCF file? by anikcropscience ▴ 230
But I used the same bam files in both freebayes and GATK. Even the overlapping SNPs from both software are giving different estimates in te…
Comment: How to get the ratio of allele counts from GATK derived VCF file? by Pierre Lindenbaum 162k
may be downsampling(?), QC failing reads ?
Comment: How to get the ratio of allele counts from GATK derived VCF file? by anikcropscience ▴ 230
Thank you. However, I extracted the AD for each SNP that gives two numbers separated by a comma. I guess the first one is for reference and…
Answer: How to get the ratio of allele counts from GATK derived VCF file? by Pierre Lindenbaum 162k
> However, I am doing SNP calling using GATK, but there is no such information field for reference and alternate allele counts. it's in …
Comment: samtools write-index by GenoMax 142k
> The index file is older than the data file: alignment.sorted.bam.bai I don't think that warning is going to cause any issues. Not sure w…
Comment: samtools write-index by Pierre Lindenbaum 162k
can you please retry, but add rm -vf alignment.sorted.bam.bai before invoking minimap2. And `-o alignment.sorted.bam` should be enoug…
Answer: from CRAM to fastq by Pierre Lindenbaum 162k
if the CRAM/BAM is paire-end, it **must** be sorted on query name using samtools collate https://x.com/lh3lh3/status/1132756684789768202 …
Comment: from CRAM to fastq by machaalani29 • 0
one of the issues is that I am not very sure how the sequencing was done, but I think it was single end. any suggestions hoot do that?
Comment: from CRAM to fastq by machaalani29 • 0
the option -c is to compress the file
Comment: from CRAM to fastq by GenoMax 142k
Possibly. You appear to have written the data to a single file (in case it was paired-end to begin with). Check to make sure secondary alig…
Traffic: 2558 users visited in the last hour
Content Search
Users
Tags
Badges
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6