Dear all,

We are excited to announce our online course “Practical Somatic Variant Analysis in Cancer Genomics”, taking place 27–29 April.

Course website: https://www.physalia-courses.org/courses-workshops/cg26/

This workshop provides hands-on training in somatic variant detection and annotation, guiding participants through the complete workflow from raw sequencing data to identification of potentially pathogenic mutations in cancer genomes. Using whole-exome sequencing (WES) data from matched tumor-normal cell lines, attendees will learn industry-standard tools and methodologies used in cancer genomics research and precision oncology.

Learning outcomes include:

• Perform QC on sequencing data and interpret metrics
• Align reads to reference genomes and assess alignment quality
• Distinguish germline and somatic variants
• Run a complete somatic variant calling pipeline using GATK4 Mutect2
• Annotate variants with VEP and interpret results in a cancer context
• Analyze Variant Allele Frequency (VAF) distributions
• Filter, prioritize, and visualize potentially pathogenic mutations

Course schedule: 2:00–5:00 PM Berlin time

Day 1: Foundations, sequencing QC, read alignment, data formats

Day 2: Somatic variant calling with GATK4, variant filtering, key resources

Day 3: Variant annotation, analysis in R, interpretation, and visualization

Best regards,

Carlo

Carlo Pecoraro, Ph.D

Physalia-courses DIRECTOR

info@physalia-courses.org