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Picard AddOrReplaceReadGroups,

Hi,

I am using Picard's AddOrReplaceReadGroups and I would like to ask the appropiate values for each field. or could I assign any names I choose?. I have fastq files and I don't know if reads identifiers contain any information that need to be included in the different fields of RGID, RGSM, RGPU, or RGPL.

human illumina libraries picard

1 answer

see "Meaning of the read group fields required by GATK"

https://gatk.broadinstitute.org/hc/en-us/articles/360035890671-Read-groups

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