More posts like this
-
RNASeq Analysis: data from different library
written by Matteo 0Hello everyone I’m trying to figure out if it’s possible to analyze NGS data from datasets obtained using different library prep strategies. Specifically, I want …
-
small RNAseq analysis of non human samples
written by luffy 13Hello all, Please share a workflow/pipeline for RNA-seq analysis focused on small RNA of insects such as roaches. Since annotation is available for protein coding …
-
Expression analysis of miRNAs with normal RNA-seq data without small RNA-seq data
written by Burak Muhammed 0Hello everyone. Can I do expression analysis of miRNAs with normal RNA-seq data without small RNA-seq data? Which path should I choose for known miRNAs …
-
Genetic variation calling (SNPs) from bulk RNA-seq dataset
written by Shicheng Guo 963Dear All, Is there anything should be careful for the workflow developed for Genetic variation calling (SNPs) from bulk RNA-seq dataset. Is there mature established …
-
get miRNA expression form total RNA data
written by aminijavad4 1HI I want to get miRNA expression from total RNA-seq. for alignment, I used HISAT2 as the aligner and mm10 as the reference genome. but …
-
Trimming Ion Total RNA-seq Kit v2
written by drsami 9Hello Everyone, I am wondering how can i find the adapters sequences to trim for Ion Total RNA-seq Kit V2 library preparation kit. If anyone …
-
select reference mir from NGS data
written by ystokar 0I have NGS data of small RNA and i would like to validate for select miRNAs using PCR. Can I use the mir-seq data to …
-
Can i do Mirna DEG with rna seq data ?
written by sabaghianamir70 7Hello Can i Extract the mirnas from my RNA seq data and then perform a mirna DEG ? or just i can do this with …
-
Mir-RNA analysis by RNA-seq data
written by lkianmehr 10Hello to all! I am gonna identify mir-RNA from RNA-seq reads, but it's the first time I am working for mir-RNA. is it same with …
-
Rna-Seq pipeline in R
written by ta_awwad 35Hello everybody, I am glad to be a member of this great blog.I am analyzing RNA-Seq data right now. I did the analysis with CummRbund …
Smaller inserts (which will include miRNA) are normally removed duing total RNA library prep. So you will likely not be able to find usable miRNA data from Total RNAseq.
Thank you