Trimming tool
Hello,
Is there any free standing tool kit to trim extra bases or adapter sequences from fastq files?
Thanks
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2 answers
There are plenty. bbduk.sh from BBMap suite (https://jgi.doe.gov/data-and-tools/software-tools/bbtools/bb-tools-user-guide/bbduk-guide/ ) , fastp (LINK), cutadapt (LINK) and trimmomatic (LINK).
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I use Trim Galore which automatically recognizes adaptors. https://github.com/FelixKrueger/TrimGalore
Here is the script I am using for paired-end sequencing
for i in $(ls *R1* | sed s/_R1.fastq.gz// | sort -u);
do
trim_galore --paired ${i}_R1.fastq.gz ${i}_R2.fastq.gz -q 20 -o ./galore-trimmed --fastqc --cores 6 --stringency 3
done
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