Can I choose the sanger sequencing?
I have a question about sequencing
Can I choose the sanger sequencing?
Suppose I would like to sequence a gene (for example, BRCA2) in order to identify both the DNA sequence and the protein sequence.
Aim: to identify each position in the dna and protein, and the type of mutation occurring ( insertion, deletion etc)
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You do appreciate that BRCA2 is a large gene (84kb) so while you could use sanger sequencing it would be rather expensive. A higher throughput method may be initially used and then the mutations found could be confirmed with sanger sequencing.
See: https://www.sciencedirect.com/science/article/pii/S1525157823001034
What are you actually aiming to show? Why do you want to use Sanger specifically?