DNA sequencing results -- How to get consensus sequence from forward and reverse strands?
I have my DNA sanger sequencing results for 5 samples in fasta format. In the results, I have the sequence of a forward and reverse strand for each sample. How do i get a consensus sequence from both strands? I need this sequence to do a BLAST search to identify sample species. Thank you
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Is this for high-throughput sequencing data in fastq format or sanger sequencing in fasta format?
Sanger sequencing in fasta
Try JalView. You should be able to generate a consensus after aligning the data.