BigWig file Normalization
Hi,
Could you guide me for the normalization of the bigwig file based on RPM should I first normalize bedgraph file and then generate bigwig or not? And which tools are appropriate?
I used this command:
bamCoverage -b SRR21230488_marked_duplicates.bam -o output.bw --normalizeUsing RPKM
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daffodil •
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