blastn Error: mdb_env_open: Cannot allocate memory
Dear All,
I downloaded nt database using wget ftp://ftp.ncbi.nlm.nih.gov/blast/db/nt.*.tar.gz it has 115 nt files.
I run blastn using a genome file to the nt database but I received the error below:
Error: mdb_env_open: Cannot allocate memory
I tried different versions of blastn (2.9.0, 2.15) but nothing changed.
The command I used is :
blastn -db ~/Databases/Blast_nt/nt -out A.genome.blast -query A.genome.fa
I tried to increase memory using the command below:
export BLASTDB_LMDB_MAP_SIZE=100000000
Nothing changed. Any solution or suggestion ?
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How much memory do you actually have? NT will require tens of GB of RAM.
I used 900 GB. I run the command on clusters.