ChipSeq and DeepTools
Hello,
I have done ChipSeq for a transcription factor and I am trying to generate a heatmap using deeptools. I am confused as to what bed file should I use for computematrix?
chip-seq
deeptools
• 1,216 views
•
link
updated
by
Ram
•
written
by
qudrat.nii •
0 answers
No answers yet.
Log in to answer this question.
More posts like this
-
Filtering transcription factor and HOMER and ENCODE
written by qudrat.nii •Hello, I have a list of transcription factors identified by HOMER tool form MACS2 peak called. I want to filter this list as it has …
-
SEACR and MACS2
written by qudrat.nii •Hello everyone, I have done cut&run for a transcription factor in a mutant cell line (mutant for mentioned transcription factor as a control). I did …
-
merging bam file
written by qudrat.nii •Hello everyone! I have done two independent chipseq for a transcription factor with two replicate in each experiment. I want to merge the bam file …
-
MACS2 , heatmap
written by qudrat.nii •Hello guys, I am doing cut&run analysis for some transcription factors and I have three replicates of BAM files and three replicates of MACS2 output. …
-
Cut&Run and heatmap
written by qudrat.nii •Hello guys, I am doing cut&run analysis for some transcription factors and I have three replicates of BAM files and three replicates of MACS2 output. …
-
Comparing two CUT&RUN (similar to ChipSeq) generated peaks
written by susan.kuo.run •Hi all, I ran a CUT&RUN experiment targeting a transcription factor in a normal control cell line and its shRNA-knockdown. I would like to prove …
-
ChIP-seq heatmap input bed file
written by buffealo •Hello, I am getting confused about the ChIP-seq heatmap concept. In particular, what should be the .bed file exactly? For example while using deeptools before …
-
Bedfile format deeptools
written by Lalita •Hello everyone, Using deeptools I am trying to plot the enrichment in Genebody and TSS for this we have to give one BED file region …
-
Deeptools Sorting for score values
written by groupelmarwolf •I am having a scored bed file with weighted values in the 4th column. I want to use this scored file to as a sorting …
-
How to generate a heatmap of a complex (three transcription factors)
written by SamIs there a way to generate a heatmap of a complex of transcription factors (in more detail, I am trying to generate a heatmap of …
Peaks, genes of interest, random regions. The entire genome. Anything that fits your story.
The bed file is whatever regions you want to look at. In your case, it may be the called TF peaks. Deeptools will then plot the signal (read counts) over each region (a line in the bed file) and present in a heatmap, where rows are the regions and color is the signal.