Hi, I tried it now with no space, but it pops up the same error. There should be another thing, ...!
Dear all,
I am stuck at one step in whihc I am trying to select SNPs or INDELS from a raw VCF file. I have used this comand
gatk SelectVariants \
-R Ref/GCF_000002315.5_GRCg6a_genomic.fna\
-V Raw_variant/ L11A_rawvariant.vcf\
--select-type-to-include SNP \
-O Raw_variant/ L11A_SNP.vcf
When I run this line, I got an error :
A USER ERROR has occurred: Illegal argument value: Positional arguments were provided ',L11A_rawvariant.vcf{L11A_SNP.vcf}' but no positional argument is defined for this tool.
Below it it says
Set the system property GATK_STACKTRACE_ON_USER_EXCEPTION (--java-options '-DGATK_STACKTRACE_ON_USER_EXCEPTION=true') to print the stack trace.
Could you please explain to me what this means. I checked the recent documentation of GATK for current version (4.4.0) also for my version 4.3.0, but it did not say anything, and it also recommend writing the code the way I have done as shown in this link for the recent GATK https://gatk.broadinstitute.org/hc/en-us/articles/13832694334235-SelectVariants
Could you please help me fix this ? What do I need to include ?
I tried to get the stack trace, by running --java-options '-DGATK_STACKTRACE_ON_USER_EXCEPTION=true' but it says
--java-options: command not found
Thanks
1 answer
-O Raw_variant/ L11A_SNP.vcf
You appear to have an extra space between the variant/ and L11. Remove that.
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