Why coordinate sort is required in sambamba depth?
When I run the command
sambamba depth base -L genomicdataset001.bed $filename
It gives the following error
sambamba-depth: All files must be coordinate-sorted
Why coordinate-sort is required in this run and what coordinate sorting is exactly?
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Pierre Lindenbaum
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MobiusT •
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what coordinate sorting is exactly?
Why coordinate-sort is required
coordinate sort is de facto the simplest way to know how many read overlap a region.
and now, review and/or validate all your previous questions: How to use minimap2? ; How a fastq.gz file is formatted? ; What is read depth exactly? ; Samtools index and sambamba-depth error? ; sambamba-depth: All files must be coordinate-sorted error? ...
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