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How to get uniprot accessions from a multi protein sequence fasta file

I have a list of proteins with amino acids in fasta format. I need to get the uniprotKB accessions for each protein. How would I go about getting these?

uniprotkb uniprot proteomics
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updated by GenoMax GenoMax • written by Lucas R.F. • Lucas R.F.

Do you have any accession numbers or are these just plain sequences with no identifiers?

If you have some identifiers then you could simply use UniProt ID converter tool: https://www.uniprot.org/id-mapping

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written by GenoMax GenoMax

2 answers

Find a protein sequence to run BLAST sequence similarity search by UniProt ID

https://www.uniprot.org/blast

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written by Pierre Lindenbaum Pierre Lindenbaum

The limit is 20 sequences whereas I have around 2,000.

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written by Lucas R.F. • Lucas R.F.

download uniprot as fasta and use a local blast.

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written by Pierre Lindenbaum Pierre Lindenbaum

If these sequences are from the same organism, you may get away with downloading just the proteome of that particular species and then doing local BLAST.

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written by Mensur Dlakic Mensur Dlakic

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