Hello, I was wondering if people might talk about their take on merging Hi-C data from different samples that are the same biologically (e.g. the …

Is there a reason why fastq files from SRAExplorer and SRAToolkit are different sizes? I am downloading paired end single-cell rna-seq data. I am getting …

Hi! Does anyone know why ENCODE's Hi-C raw data (FASTQ files) are single-end? See for example here: https://www.encodeproject.org/files/ENCFF846THP/ and the corresponding Sequence Read Archive Entry: …

Hi! Does anyone know if someone has created a resource or gathered information about which type of layout each sequencing machine produces? Some table or …

Hi, I was wondering if anyone can confirm this: one Sequence Read Archive Run ID always corresponds to one single-end FASTQ file or one pair …

Hi! I was wondering if anyone knows what the current best practices are for QC and trimming sequencing bulk RNA-seq reads before aligning them to …

Hi friends! I come seeking wisdom. when is it appropriate to do a [dotplot][1] with your single-cell data? I've seen people plot "by hand" genes …

Hi, I was wondering if someone knows what would happen in this case: - rule_a in my snakemake workflow has wildcards in its output ("output_{type}.txt") …

Hi, I installed the sratoolkit from NCBI by following. While running prefetch, the following error generated. I assume something wrong with my path. How can …

Hello, I really don't understand why the same gene has a different number of transcripts, but moreover, a different start position. In this case for …