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Transcript Abundances Meta-transcriptome

Hello! I am trying to learn the procedure for meta-transcriptome analysis.

I am not able to understand

  1. How the transcript abundances will be calculated after de-novo assembly?
  2. How can the read counts be normalized and Differential Expression estimated? Please suggest suitable tools.

I would also like to request some easy study materials for understanding the entire process better.

Please help.

Rgrds, Akash

transcript meta-transcriptome abundances rna-seq

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