Coverage Depth Transcriptome Assembly
I am trying to estimate coverage depth for my de novo transcriptome assembly. I used Illumina Hiseq 3000/4000 PE with a PF yield of 140 million clusters. Since the organism hasn't been studied before I am having trouble finding the total number of transcripts. Can someone tell me of a way to calculate the coverage depth. Will I have to calculate the coverage depth on average for each transcript or can I get a coverage depth for the entire transcriptome? Thank you
• 1,385 views
•
link
written
by
babji2318 •
0 answers
No answers yet.
Log in to answer this question.
More posts like this
-
Calculating coverage/depth for nanopore dRNAseq reads with reference transcriptome/genome
written by kenneditodd •
-
Extracting transcripts from whole-transcriptome libraries
written by julianneradford •
-
Post Alignment basic QC of RNA-Seq data
written by daewowo •
-
Number of passing clusters vs. number of read pairs vs. total number of reads
written by AP
-
How to calculate average depth for each Amplicon in targeted amplicon sequencing?
written by bioinfo89 •
-
How to calculate the average of mapping coverage?
written by seta
-
Bcl to fastq conversion problem in bcl2fastq-2.17
written by BioRyder
-
How compare two de novo assembled transcriptomes of the same sample with different sequencing depth?
written by seta
-
Chip Sequencing For A Tiny Genome
written by André Rendeiro •
-
What Is Best Strategy To Use Miseq 2X300 Reads To Improve De Novo Transcriptome Assembly Based On H…
written by lzwright
