Extracting transcripts from whole-transcriptome libraries
Hi there,
I am having trouble finding a tool that will successfully extract specific transcripts from whole transcriptome libraries. The transcriptome libraries were assembled through Trinity, and the reference transcriptome was a de novo assembly from Trinity also.
My ultimate goal is to extract the transcripts of interest, and then compare SNP counts and calculate dN/dS ratios. Any help is appreciated. Thanks.
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julianneradford •
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Define specific. Particular proteins/genes? In any case you will have to identify a reference set by annotating one library and then you can identify transcripts of interest from others by sequence searching.
By specific I mean genes, yes. I have 9 fastq transcriptome libraries that have been mapped to an annotated reference transcriptome, and I generated VCFs as well. I would like to pull the same specific genes (each sequence ("gene") is labelled with it's trinity identifier) from each of the fast q libraries. Do you know of a tool that sequence searches and pulls out specific sequences from either BAM or VCF files?