Hello,

I am doing an RNA-Seq study on a non-model organism that has a published transcriptome, but no genome and no GTF file. If I were to use De Novo assembly (for example, trinity tools) is there a way to generate a GTF file? I understand that I can use tools like Bowtie, RSEM, and/or EdgeR for transcript abundance and quantification after the de novo assembly - is it possible to create a GTF file this way?

Thank you!