Is it normal that my amplicon sequence variants (ASVs) have gaps ?
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22 months ago

Hello my chief sent me some working files to perform a functional analysis from amplicon sequences. (from 16S rRNA genes)

This is the first time that I perform an analysis from amplicon sequences so I want to know if from the DADA2 pipeline it is normal to output a fasta file with ASVs with gaps inbetween the sequences like this:

fasta example

Thanks for your time :)

ASVs DADA2 fasta • 338 views
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