Is it possible to find the mutation in RNA-seq reads?
Hi all,
I am analysing the CLIP-Seq data. The protocol follows UV treatment to identify the protein RNA-interaction so i am introducing a mutation. So my question is there any way to identify these mutations. If the question is not clear please ask me?
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Carlo Yague
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PK •
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PK •
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The mutation footprint induced by the UV-crosslink in CLIP and PAR-CLIP experiments is used as a proxy for protein binding in multiple tools, including:
I suggest that you read the papers and try to understand how they identify the mutations. If you have more specific questions, feel free to come back and ask for clarifications.
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