Bowtie2 align
I am using bowtie2 to align . But after indexing, while I am running the bowtie2 , I am getting the following error. Can anyone help me? I need to know.
bowtie2 -x Baumannii_indexed.btindex -f -U M003786.fasta -s aligned.sam
stat: Bad file descriptor
Warning: Could not open read file "M003786.fasta" for reading; skipping...
Error: No input read files were valid
(ERR): bowtie2-align exited with value 1
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Error says
Is that file in the directory you are running this command from? What does
head -3 M003786.fastashow?Sorry. I have given the wrong file name. But when I have done everything ok, now this is the problem showing
Re-posted here: bowtie2