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Hisat2 / (ERR): hisat2-align exited with value 1

Hello I'm trying to use Hisat2 on WSL, and I used -wdp founding location of indexs (hg19 downloaded), fastq file and output.sam. But:

Warning: Could not open read file "/mnt/c/Users/.../trimmed_out.fastq" for reading; skipping...
Error: No input read files were valid
(ERR): hisat2-align exited with value 1

What should I do? Thanks for your reply


Thanks, all things are OK. problem solved.

alignment software error

Which was your command? Can you share (please)?

Thanks, all things are OK. problem solved.

Error was clear originally so I assume once you addressed the issue the problem went way? If you did something else then please post that an answer to provide closure for this thread.

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