Dear all,

I hope all of you be fine and healthy.

I merged two count matrices, after merging and batch effect removal by the ComBat_seq function, I do not see a detectable change in batch effect removal.

count_matrix <- merge(batch1, batch2, by= "Symbol")
batch <- c(rep("1", 14), rep("2", 22))
adjusted <- ComBat_seq(count_matrix, batch=batch, group=NULL, full_mod=FALSE)

How can the batch effect be completely removed?

I would be grateful if you could guide me to resolve it.