Hello. **Background:** - I have to assemble some fungal genomes, which are known to have accessory chromosomes. - Initially we tried `Nanopore 1D + Illumina …
Hi, AUGUSTUS comes with a script which can filter BAM files filterBam –uniq –paired –pairwiseAlignment –in Aligned.out.ss.bam –out Aligned.out.ssf.bam Does StringTie does any filtering by …
New tools have been published for error correcting PacBio reads with Illumina reads: - HALC (https://bmcbioinformatics.biomedcentral.com/articles/10.1186/s12859-017-1610-3 ) - FMLRC (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5807796) I am just wondering which …
Hi, I ran dextractor (https://github.com/thegenemyers/DEXTRACTOR) and it generated X.subreads.arrow and a FASTA file. How could I polish the reads before the assembly? Thank you in …