Thank you very much for the information you gave me. Quiver seems to be the best choice, I will try it!
Use PacBio FASTQ reads to polish\correct a PacBio Fasta assembly without Quiver
Hi!
I have assembled my PacBio FASTQ reads with Canu. Now I would like to polish\correct the assembly by mapping these PacBio FASTQ reads on the assembly itself. I heard about Quiver in the SMRTanalysis pack, but I'm wondering if there is any alternative software.
Thanking you in advance for your help!
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Canu uses alignments between pacbio reads to correct away most sequencing errors in raw reads. You can't do much better without using the signal information. To achieve high-quality consensus, Quiver is your only choice.
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Thanks for your answer, It was really useful!
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