Hi,
I was wondering whether anyone knows of a good blog/video/post explaining sctransform in a clear and easy to understand way. I understand the idea of the approach but would like to further understand the details. The paper uses a lot of unfamiliar terms which makes it tricky to read.
Alternatively, if anyone wants to have a go at explaining it, I would much appreciate it!
Best wishes,
Lucy
2 answers
Florian Wagner has a great (and short) video explaining different scRNA-seq preprocessing methods here. The "the Pearson residuals from regularized negative binomial regression" approach implemented in scTransform is one of the approaches he explains in a very clear and understandable way.
I found this document quite informative about the rationale and process of the transformation.
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