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MGEs analysis in metagenomic data by ACLAME data base

Hello everyone.

I want to analyze MGEs from metagenomic data and i already trimmed adaptors sequences by scythe and trimmomatic and got my clipped fasta file. I want to analyze MGEs by ACLAME database. For that i need your guideline that how i can do blast by using ACLAME database. Please guide... Thanks!

mge aclame

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