the relationship of ATAC-seq and H3K27ac
Hello,
ATAC-seq/DNase-seq are mainly used to identify open chromatin regions/accessible chromatin. H3K27ac is a active enhancer marker. Does anyone know what the relationship of open chromatin regions and H3K27ac? Do all H3K27ac binding sites overlap/intersect with open chromatin regions?
2 answers
"elements with strong DNase-seq signal but no H3K27ac ChIP–seq signal might be CTCF-bound topological elements, and elements with strong H3K27ac signal but no DNase-seq signal might be sequences that are close to strong enhancers, but do not per se have enhancer activity due to the spreading H3K27ac signal over hundreds to thousands of base pairs"
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