This is a test version of Biostars. For the public version, visit https://www.biostars.org.
Finding adapters TruSeq Stranded Total RNA with Ribo-Zero H/M/R_Gold

Hi,

I have some fastq files and I want to remove adapters. The kit used was "TruSeq Stranded Total RNA with Ribo-Zero - H/M/R_Gold" but I can't find any info on it. Anyone know where I can find the adapters used for this kit?

rna-seq fastq illumina
• 2,722 views • link
updated by Istvan Albert Istvan Albert • written by newb • newb

1 answer

  1. fastp will identify possible adapters in your data automatically.
  2. You could use bbmerge method - How to figure out adapter sequence for Illumina reads?
  3. These should be standard TruSeq adapters. You can find the sequence in Illumina's site here.
• 0 views • link
written by GenoMax GenoMax

Thanks a lot for your response!

There are several TruSeq kits on Illumina's site and they don't seem to all have the same kind of adapters. Do you know which kit I should look at?

• 0 views • link
written by newb • newb

Great, Thanks a lot for the help!

• 0 views • link
written by newb • newb

Log in to answer this question.

More posts like this