Poly(A) Tail In Rna-Seq Data
I was just wondering if a) the protocol for RNA-Seq removes/modifies the mRNA Poly(A) tail, and if not: b) RNA-Seq can read a fragment which includes part (or all) of the the Poly(A) tail c) which file type would include data on the reads including the Poly(A) tail
Thanks!
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Istvan Albert
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chocho •
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chocho •
1 answer
I can't speak of all protocols but most commonly the poly A tail is present and need to be removed with a trimming program.
See for example
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