I want to just give a snapshot to type of sequencing and their main use. This post is for beginners who are into NGS data analysis.

1. Whole Genome Sequencing (WGS):

a. Sequencing entire chromosome in the organism which includes introns, exons, UTR etc

b. Mainly used for variant calling analysis on all part of the genome

2. Whole Exome Sequencing (WES)

a. Sequencing of all coding regions (exons) in the genome

b. Variant calling analysis is identify variants in the coding regions

3. Chip-seq

a. Used for identification of epigenetic changes which include identification transcription factor, methylation, protein-DNA interaction

4.RNA-Seq

a. Mainly used for identification transcript expression, novel coding regions, novel isoforms etc.

b. Used for variant analysis in the expressed region