Poly(A) Tail From Rna-Seq?
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I am not certain of good responses to your two questions, but I have read that length of the poly(A) tail can vary according to conditions within the cell. Thus, noting the length of poly(A), if you have accurate measures from RNA-Seq, between control and various conditions may be an important characteristic of your gene expression data to retain.
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Larry_Parnell
I have seen poly A reads in my own limited data.
The following paper has specifically looked at poly A reads as a way to get alternative poly A sites
http://www.nature.com/nature/journal/v464/n7289/full/nature08872.html
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