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Why 3' End Has A Lower Quality In Ngs Data

Hi all,

I observed that NGS sequence reads have a lower quality towards the 3' end. I would like to know the reason for having low quality reads towards 3' end. Hope someone can help me.

Thank you inadvance

ngs
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updated by Ashutosh Pandey Ashutosh Pandey • written by Kash Kash

1 answer

Check this post on a different forum

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updated by Devon Ryan Devon Ryan • written by Ashutosh Pandey Ashutosh Pandey

Sorry to bother you again but I can not access this link. Can you please copy the content of thread here

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written by Kash Kash

I've updated ashutoshmits's answer with the correct link. The gist is that there is an accumulation of errors and noise over time (the same phenomenon occurs in sanger sequencing).

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written by Devon Ryan Devon Ryan

Thank you very much. I got the point

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written by Kash Kash

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